My First Successful Ligation

Every first-time is just as important, this applies to my first ligation, too! Click on the link to check what the hell does ligation mean. Now, after knowing what ligation is, you must be wondering what's the big deal about it - it's just putting the enzymes and different DNA fragment together in a tube. No BIG deal, you're right.

The catch is, the probably of successful ligation is soooooo darn low, so low that some people take THREE months (If your luck is so suay, it may be longer) to get it done. If you failed, it'll take you AT LEAST 3 days to PREPARE for the next experiment. It's very time consuming and tedious.

After numerous failures, you'll do all sorts of funny things like casting spells and drawing lucky charms to make sure your stuffs work.

My agar plate on the right and Mabel's on the left.

And by the time you get it done properly, you need to transform the recombinant DNA into a new cell host. Easy as it sounds... The thing is, you wouldn't know whether your failure is caused by ligation or transformation untill you grow your newly transformed cells on agar plate - having colonies means there were ligations but it doesn't mean they are the correct ligation. This will take another day... (4 days till now)

See the white spot? That's a COLONY!!

Take a closer look on my babeh!

If you're so lucky that you've colonies appear on your plate (assuming that your charms and spells work) then you'll extract the DNA out and test if they are the correct ones or not. This will take ANOTHER day... (total of 5 days) If you fail the test, you have to repeat the WHOLE THING AGAIN.

Woo hooo... Ligation is soooo FUN!

I know you don't give a damn about this picture, I just wanted to shout to the whole world that all my colonies are transformed with the CORRECT recombinant DNA.
*peace sign*

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

Danga Bay Cruise

Just some picture of the past...

Synergy cell mates

The Singapore Woodlands Immigration Building

Needless to say

This is how an OMG OMG OMG butter crayfish looks like

And we consumed everything... CONSUMED! That includes the butter and curry leaves =..=
See? It's so good, right?

Taken on 13th Dec, 2008. Danga Bay Cruise - TCC YAS Event
Makan place: Jalan Keris, Taman Sri Tebrau (Nearby Crystal Crown Hotel), Johor, Malaysia.

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

The Minus -Eighty-Degree-Celsius Freezer

There is a minus-eighty-degree-Celsius freezer hiding in a corner of NTU. (OK, its at NTU-SCBE-CBE-B3-30. Come and steal it.)

Mind you, if you are thinking of stealing this baby, remember to wear a piece of high-quality hand glove. Moboto.blogspot.com shall not be responsible if your hand got deep froze and detaches itself from your arm.

Behold, it's the all mighty minus-eighty-degree-Celsius freezer!
Hold your breath and get ready to kowtow!

Foooo!!! Feel the wrath of the Ice Queen Monster!

I was almost swallowed up by the Ice Queen Monster while taking this up close picture for my readers. Sadly, my relentless efforts are always gone unappreciated.

The Chinese says, "You won't get the cub if you don't enter the tigers' dent"
Here I come! Ice Queen Babies!

Gotta be quick! Before she realizes...

I grabbed the Ice Queen Babies from the nursing cell at the speed of light.
ZOOOP!

Ahh... there you are, my baby~

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

My Pee Pee Scan

If you are wondering what this is, it's the separation result of my pee pee by Ultra High Performance Liquid chromatography. Understand? No? So am I. =)

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

NTU-SCBE-CBE Lobby X'mas Deco

I know this is a darn outdated entry... but who cares~

Lovely X'mas tree standing alone in the lobby of NTU psychotic ward CBE faculty

with some empty presents from Santa
(this is how our school fund gone missing - they go shopping, claim under the school fund, take their stuffs and wrap the box)

-with shooting stars-
I have a dream, a song to sing;
to help me grow, in everything...

And Candy Canes!!
Legends say that the sweet was not model after a cane but the letter J.
J for Jesus =)

We can't afford to buy a real pear tree, neither an ordinary fake pear tree.
But it's okay, we have a micro version.

Same applies to lemon tree, too!

Wishing you a Merry 2009 X'mas in advance!!

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

Somewhere inside My Kitchen

Hi folks! I'm Palastine, the retard standing on my right is Israel and I hate him.

Mom loves Israel more than me. She feeds him with sugar while stuffing me with thin air. We quarrel almost every single day for territory rights in our shelve but Mom always throw me into the back of Soy Sauce whenever Israel complaints to her.

But we learn how to live with it. We don't fight and we don't push each other down the shelve because we know we are part of the Kitchen. The kitchen needs us and we need the Kitchen.

Therefore, we learn to accommodate one another for the sake of the Kitchen.


Heal the Kitchen,
make it a better place
for you and for me
and the entire Kitchen race.

There are people eating
if you care enough
for the food tasting.

Make a better place
for you and for me.


STOP THE WAR!

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

Israeli Assault on Gaza

OK, Israeli assault on the Gaza is nothing new now. It has lasted for about 2 weeks since the first air strike. Now, let's see how U.S. reacts to the issue... Quoted from Channelnewsasia.

US President George W. Bush, in remarks to be broadcast on Saturday, urged all able parties to press Hamas to stop firing rockets at Israel and secure a lasting ceasefire, after a week of heavy Israeli air strikes on the Gaza Strip.

His administration meanwhile gave Israel free rein over whether to send ground troops into Gaza, despite growing criticism over its handling of a conflict that has killed at least 436 Palestinians and left 2,290 others wounded...

...Bush said the Israeli strikes were in self-defense after Hamas let a six-month ceasefire lapse on December 19 and fired rockets at Israel...

Zbigniew Brzezinski, who was national security adviser for US president Jimmy Carter, told CNN television that Rice's remarks "clearly show that the US policy right now is completely bankrupt" and the Israeli offensive "will further radicalise the Palestinians"...

...But the US president-elect has kept silent on the latest phase of the Israeli-Palestinian conflict, with his aides saying their boss was "monitoring" the situation.

So, it's like a child's game la. I slap your face, then you slap me back. I wanna have a greater revenge, I ask my big brother to slap you again. You wanna have an even greater revenge, but obviously you are nothing compared to the fist of my bigger brother, you start throwing stink bomb into my house. And the game goes on and on...

With regards to this matter, I have a song to every reader to this blog (Well, probably none,but it's okay). What a nice way to start my first entry of the year...

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::