My First Successful Ligation

Every first-time is just as important, this applies to my first ligation, too! Click on the link to check what the hell does ligation mean. Now, after knowing what ligation is, you must be wondering what's the big deal about it - it's just putting the enzymes and different DNA fragment together in a tube. No BIG deal, you're right.

The catch is, the probably of successful ligation is soooooo darn low, so low that some people take THREE months (If your luck is so suay, it may be longer) to get it done. If you failed, it'll take you AT LEAST 3 days to PREPARE for the next experiment. It's very time consuming and tedious.

After numerous failures, you'll do all sorts of funny things like casting spells and drawing lucky charms to make sure your stuffs work.

My agar plate on the right and Mabel's on the left.

And by the time you get it done properly, you need to transform the recombinant DNA into a new cell host. Easy as it sounds... The thing is, you wouldn't know whether your failure is caused by ligation or transformation untill you grow your newly transformed cells on agar plate - having colonies means there were ligations but it doesn't mean they are the correct ligation. This will take another day... (4 days till now)

See the white spot? That's a COLONY!!

Take a closer look on my babeh!

If you're so lucky that you've colonies appear on your plate (assuming that your charms and spells work) then you'll extract the DNA out and test if they are the correct ones or not. This will take ANOTHER day... (total of 5 days) If you fail the test, you have to repeat the WHOLE THING AGAIN.

Woo hooo... Ligation is soooo FUN!

I know you don't give a damn about this picture, I just wanted to shout to the whole world that all my colonies are transformed with the CORRECT recombinant DNA.
*peace sign*

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

The Minus -Eighty-Degree-Celsius Freezer

There is a minus-eighty-degree-Celsius freezer hiding in a corner of NTU. (OK, its at NTU-SCBE-CBE-B3-30. Come and steal it.)

Mind you, if you are thinking of stealing this baby, remember to wear a piece of high-quality hand glove. Moboto.blogspot.com shall not be responsible if your hand got deep froze and detaches itself from your arm.

Behold, it's the all mighty minus-eighty-degree-Celsius freezer!
Hold your breath and get ready to kowtow!

Foooo!!! Feel the wrath of the Ice Queen Monster!

I was almost swallowed up by the Ice Queen Monster while taking this up close picture for my readers. Sadly, my relentless efforts are always gone unappreciated.

The Chinese says, "You won't get the cub if you don't enter the tigers' dent"
Here I come! Ice Queen Babies!

Gotta be quick! Before she realizes...

I grabbed the Ice Queen Babies from the nursing cell at the speed of light.
ZOOOP!

Ahh... there you are, my baby~

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

NTU-SCBE-CBE Lobby X'mas Deco

I know this is a darn outdated entry... but who cares~

Lovely X'mas tree standing alone in the lobby of NTU psychotic ward CBE faculty

with some empty presents from Santa
(this is how our school fund gone missing - they go shopping, claim under the school fund, take their stuffs and wrap the box)

-with shooting stars-
I have a dream, a song to sing;
to help me grow, in everything...

And Candy Canes!!
Legends say that the sweet was not model after a cane but the letter J.
J for Jesus =)

We can't afford to buy a real pear tree, neither an ordinary fake pear tree.
But it's okay, we have a micro version.

Same applies to lemon tree, too!

Wishing you a Merry 2009 X'mas in advance!!

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

Very Good

I've just got back my mid-term test and guess what....
My dear dear prof commented: V. Good!
I am so freaking happy =)

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

NDP Fireworks

OK la, I am lazy to write down all my feelings, will update it someday later.

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::

So-long-ago Farewell Party

Yeap yeap, it's a long-ago-picture from Aug 22, 2008, but come on folks! I am a freaking busy man, you know! It was Rachael's farewell party after working in Singapore for two months - time really flies like flies huh?

Rachael, Regina and Zhi Rui

The Longbar is the birth place of Singapore Sling and first served in 1915. What is this Singapore-sling-around thing? The ingredients are gin, Cointreau, cherry brandy, Dom Benedictine, pineapple juice, Grenadine, Angoustura bitters and limes. However, I suggest having some beer before drinking the sling, or rather, try to mix a little of soda into the drink. It'll definitely taste better that way.

Yeap, we chilled out at the Long Bar, tried out Singapore Sling (the Long Bar invented it), eating peanuts, throwing peanuts shells anywhere you like and I love it :) What to do? After all, I am a Malaysian who like to throw rubbish everywhere and anywhere.

And we went to Zouk out after that...
Yeah yeah, I know I am very zombie that night...

All the best to you!

source: Uniquely Singapore

The Broken Tooth says
::So long, farewell; I hate to say Good Bye::